Next-Generation Sequencing: Targeting Targeted Therapies

Next-Generation Sequencing: Targeting Targeted Therapies.

Next-generation sequencing (NGS) has given new perspective in oncology. With the ongoing development of targeted therapies, NGS is evolving molecular diagnostics by providing comprehensive interrogation of clinically actionable genomic aberrations in tumors. Having this assay as the primary testing method produces clinically beneficial results. See related article by Drilon et al., p. 3631.

Bio-Informatics - Next Generation Cancer Targeted Therapies

Since its birth in the 1980s, bio-informatics has been rapidly growing, keeping pace with the expansion of genome sequence data. Latest technological development of large-scale gene expression analysis using DNA micro-arrays and proteomics experiments has further boosted the importance of bio-informatics methods. In Bio-Informatics a lot of tools & algorithms are developed to study detailed sha...

Practice guidelines for Targeted Next Generation Sequencing

DNA sequencing is the most commonly used approach for mutation scanning and is widely regarded as the gold standard. Agreed practice guidelines for both the sequencing process and data analysis are important to achieve a high quality approach, with common quality standards across different laboratories. These guidelines do not constitute an experimental protocol or troubleshooting guide, rather...

Targeted Next-Generation Sequencing for Forensic Genomics

Next-generation sequencing of adrenocortical carcinoma reveals new routes to targeted therapies

AIMS Adrenocortical carcinoma (ACC) carries a poor prognosis and current systemic cytotoxic therapies result in only modest improvement in overall survival. In this retrospective study, we performed a comprehensive genomic profiling of 29 consecutive ACC samples to identify potential targets of therapy not currently searched for in routine clinical practice. METHODS DNA from 29 ACC was sequen...